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Oral dose assessment of a disinfectant to prevent horizontal
infection and mortality in experimental fowl typhoid
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Y. D. HUBERMAN and H. R. TERZOLO
INTA EEA Balcarce, Animal Production, Bacteriology, CC 276, 7620,
Balcarce, Argentina. |
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E-mail:yhuberman@balcarce.inta.gov.ar |
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An experimental fowl typhoid infection was carried
out to determine the most appropriate dose in drinking water of the
disinfectant N-alkyl dimethyl benzyl ammonium chloride (TIMSENTM),
assessing:
- Prevention of horizontal infection
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Click para ampliar |
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Methodology
Animals: 390 Salmonella
free, 1-day-old male chickens of commercial brown layer (Gy-Brown).
Bacteriology
- Culture media: MacConkey agar
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Experimental design (Table 1)
- 6 groups (A, B, C, D. E and F) of 65 birds
each.
- Each group have 30 inoculated chickens
together with 35 non-inoculated ones.
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Treatment with TIMSENTM:
- From the 20th
day of life until the end of the trial.
- Mode of application: in drinking water.
- Doses per group (Table 1)
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Challenge
- Strain: Salmonella gallinarum
strain INTA 91/91.
- Dose: 109
colony-forming units per chicken at the 21st
day of life.
- Route: By gavage into the crop.
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Autopsies at the:
- 5th day
post-challenge: 10 inoculated and 10 non-inoculated chickens per
group.
- 8th day
post-challenge: All remaining chickens.
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Graph 1. Click to enlarge
Graph 2. Clikc to enlarge |
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Table 1: Experimental Groups
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Results
- TIMSENTM
was efficient in preventing horizontal infection at the doses of 25,
50 and 100 ppm
(Graph 1).
- TIMSENTM
at 50 ppm had significantly reduced the mortality in comparison with
the control (Graph 2).
- Doses higher than 50 ppm of TIMSENTM
resulted in higher mortality rates (Graph 2).
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Conclusions
- Administration of doses not higher than
50 ppm in drinking water is recommended to prevent horizontal
infection and to reduce mortality.
- Higher doses may cause a deleterious
outcome.
- Disinfectants should be administered
according to the manufacturer's instructions.
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