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Protection conferred by a live Salmonella Enteritidis vaccine against S. Enteritidis during the period of pullet rearing

Y. D. HUBERMAN and H. R. TERZOLO
INTA EEA Balcarce, Animal Production, Bacteriology, CC 276, 7620, Balcarce, Argentina.

E-mail:yhuberman@balcarce.inta.gov.ar

Four trials were carried out in laying pullets to evaluate the protection of a live attenuated Salmonella Enteritidis vaccine, Tad Salmonella Vac E® (Lohmann Animal Health, Germany) against S. Enteritidis challenge.

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Methodology

Chickens: Salmonella free 1 day-old commercial layer female chicks. (Lohmann Brown)

Vaccination route: By gavage into the crop.

Vaccination dose: 1 to 5 x 10⁸ Colony Forming Units per dose.

Culture media: Brilliant Green plus Nalidixic acid agar and Tetrationate broth.

Faecal samplings: 5 times by cloacal swabbing.

Organs cultured: Livers, ovaries, spleens and caeca.

Experimental design

Trials 1, 2 and 3: 42 vaccinated chickens and 42 unvaccinated ones.

Trial 4: 68 chickens were divided into 4 experimental groups of 17 chickens each

Group 1: Vaccinated at the 1^st day of life

Group 2: Vaccinated at the 7^th week of life

Group 3: Vaccinated at the 1^st day and 7^th
week of life

Group 4: Control unvaccinated.

Challenges Dose: 2 - 5 x 10⁹ Colony- Forming Units per dose.

Strain: Virulent S. Enteritidis 285/94 Nalidixic acid resistant.

Trial 1: 6^th week of life.

Trial 2: 8^th week of life.

Trial 3: 12^th week of life.

Trial 4: 16^th week of life.

At the 5^th day post challenge: 4 - 5 chickens per group were sacrificed.

At the 15^th day post challenge: All remaining chickens were sacrificed.

Results

Fewer vaccinated chickens excreted S. Enteritidis in comparison to unvaccinated ones.

Fewer organs were found to be positive for the presence of S. Enteritidis.

Conclusions

Administration of one oral dose of Tad Salmonella Vac E® at the 1^stday of life protects the chicken at least until the 8^th week of life.

Two oral doses at the 1^stday and 7^th week of life of Tad Salmonella Vac E® during the period of pullet rearing are recommended to afford protection against S. Enteritidis infection during the rearing period.

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