|
|
Inicio >
Información > Pósters
|
|
| |
|
In vitro growth-inhibition of chicken bacteria pathogens by
N-alkyl dimethyl benzyl ammonium chloride
|
|
|
|
|
|
|
|
|
Y. D. HUBERMAN1,
D. J. BUENO2,
and H. R. TERZOLO1
1INTA EEA Balcarce, CC 276, 7620,
Balcarce, Argentina.
2INTA
EEA Concepción del Uruguay, CC 6, 3260,
Entre Ríos, Argentina.
E mail:yhuberman@balcarce.inta.gov.ar
|
|
|
|
|
|
|
|
Click to enlarge
|
|
The purpose of this work was to determine the
in vitro growth-inhibition of the disinfectant
N-alkyl dimethyl benzyl ammonium chloride (TIMSENTM)
against poultry pathogenic bacteria.
Aetiology agents of Infectious Coryza, Fowl Typhoid, Paratyphoid
Salmonellosis and Fowl Cholera were selected together with other
associated chicken pathogens.
Methodology
Bacteria: Nineteen strains isolated from
chickens.
Bacterial concentration: Different
bacteria concentration with a minimum of 106
colony forming units (CFU) per mL (Table).
|
|
|
|
|
|
|
|
|
Click on the picture to enlarge |
|
Culture media:
- Avibacterium paragallinarum
strains in Columbia agar base plus 7% equine defibrinated haemolysed
blood.
- All the other strains in Columbia agar
base plus 7% bovine defibrinated non-haemolysed blood.
|
|
|
|
Doses of TIMSENTM
tested4 0 ppm
- 0 ppm
- 25 ppm
- 50 ppm
- 100 ppm
- 200 ppm
- 400 ppm
- 800 ppm
- 1600 ppm
|
|
|
|
|
Sampling times:
- Less than 1 minute
- 2 minutes
- 5 minutes
|
|
|
|
|
Results
- Complete inhibition after 5 minutes by 200
ppm.
- Complete inhibition after 2 minutes by 400
ppm.
- Complete inhibition after <1 minute by 800
ppm(*)
(*)Except S. Enteritidis PT4, which was completely inhibited only
after 2 minutes or <1 minutes with 1600 ppm.
|
|
|
|
|
Conclusions
- The manufacture's recommended dose of 400
ppm is satisfactory for complete elimination of bacteria within 2
minutes.
- 400 ppm is suitable for environmental
disinfection of clean materials and surfaces.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
