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Antifungal effect of N-alkyl dimethyl benzyl ammonium chloride
against Aspergillus fumigatus and A. flavus strains
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D. J. BUENO1, Y. D.
HUBERMAN2, and H. R. TERZOLO2
1INTA EEA Concepción del Uruguay, CC
6, 3260, Entre Ríos, Argentina. 2INTA
EEA Balcarce, CC 276, 7620, Balcarce, Argentina.
E-mail:dantejb@yahoo.com.ar
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Aspergillosis, is an infection caused by
Aspergillus fumigatus and less often by other
Aspergillus species. It is a common disease of poultry,
often following the inhalation of spores from contaminated feed,
litter, incubators and hatcheries.
Objective
The antifungic effect of the disinfectant N-alkyl
dimethyl benzyl ammonium chloride (TIMSENTM)
on Aspergillus fumigatus and A. flavus
strains was evaluated. Methods
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Click to enlarge |
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Strains: 9 Aspergillus fumigatus
and 14 Aspergillus flavus isolated from lungs of
chickens from commercial hatcheries
Culture media and growth conditions:
Potato Dextrose Agar at 37°C during 5-7 days
Concentration of spores: 106
spores/mL
Inhibition test: well agar diffusion assay.
Concentration of TIMSENTM:
0, 100, 200, 400, and 800 ppm.
Interpretation: Inhibition was considered
if there was a complete clearing halo zone of more than
1 mm around the well. |
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Results
All strains were inhibited by either 400 or 800
ppm.
Sixteen strains were inhibited by the dose of 200
ppm.
Four strains were even inhibited by a lower
concentration of 100 ppm.
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Table 1: Radial growth inhibition by the
disinfectant N-alkyl dimethyl benzyl ammonium chloride (TIMSENTM)
against Aspergillus fumigatus and A. flavus
strains*. |
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Concentration of TIMSENTM
(ppm) |
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Fungi |
0 |
100 |
200 |
400 |
800 |
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A. fumigatus XVI |
R |
ND |
S |
S |
S |
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A. fumigatus XVIII |
R |
R |
S |
S |
S |
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A. fumigatus XIX |
R |
ND |
S |
S |
S |
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A. fumigatus XX |
R |
ND |
S |
S |
S |
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A. fumigatus
266G1 |
R |
R |
S |
S |
S |
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A. fumigatus
P267 |
R |
S |
S |
S |
S |
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A. fumigatus
P271 |
R |
ND |
S |
S |
S |
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A. fumigatus
P505 |
R |
S |
S |
S |
S |
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A. fumigatus
CC |
R |
R |
R |
S |
S |
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A. flavus I |
R |
ND |
R |
S |
S |
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A. flavus II |
R |
R |
R |
S |
S |
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A. flavus III |
R |
ND |
S |
S |
S |
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A. flavus V |
R |
R |
S |
S |
S |
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A. flavus VI |
R |
S |
S |
S |
S |
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A. flavus VII |
R |
R |
R |
S |
S |
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A. flavus VIII |
R |
R |
S |
S |
S |
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A. flavus IX |
R |
R |
S |
S |
S |
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A. flavus X |
R |
S |
S |
S |
S |
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A. flavus XI |
R |
ND |
S |
S |
S |
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A. flavus XI 20 |
R |
R |
S |
S |
S |
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A. flavus XIV |
R |
ND |
R |
S |
S |
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A. flavus XVII |
R |
ND |
R |
S |
S |
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A. flavus CC |
R |
R |
R |
S |
S |
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*S: Sensible; R: Resistant; ND:
Not Done. |
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Conclusions
The manufacture's recommended dose of 400 ppm
is satisfactory for environmental disinfection against
Aspergillus and for the prevention of aspergillosis in
hatcheries and poultry farms. |
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REFERENCES
Kunkle, R. 2003. Fungal infections. In: Diseases
of Poultry, 11th ed. Y. M. Saif, H.
J. Barnes, J. R. Glisson, A. M. Fadly, L. R. McDougald, and D. A.
Swayne, eds. Iowa State University, Press, Ames. pp. 883 902.
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